Sequencing 5-Hydroxymethyluracil at Single-Base Resolution
نویسندگان
چکیده
منابع مشابه
Quantitative sequencing of 5-methylcytosine and 5-hydroxymethylcytosine at single-base resolution.
5-Methylcytosine can be converted to 5-hydroxymethylcytosine (5hmC) in mammalian DNA by the ten-eleven translocation (TET) enzymes. We introduce oxidative bisulfite sequencing (oxBS-Seq), the first method for quantitative mapping of 5hmC in genomic DNA at single-nucleotide resolution. Selective chemical oxidation of 5hmC to 5-formylcytosine (5fC) enables bisulfite conversion of 5fC to uracil. W...
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متن کاملSequencing the sixth base (5-hydroxymethylcytosine): selective DNA oxidation enables base-pair resolution.
the family of ten-eleven-translocation (TET) oxygenases converts 5mC to 5hmC. Last year, the two more highly oxidized congeners of 5hmC, 5-formylcytosine and 5-carboxycytosine (5fC and 5caC respectively), were also discovered as 5mC TET oxidation products. The race to disentangle the roles that these “new” DNA bases play, both as epigenetic markers and as intermediates in demethylation pathways...
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ژورنال
عنوان ژورنال: Angewandte Chemie International Edition
سال: 2018
ISSN: 1433-7851
DOI: 10.1002/anie.201804046